The governor said in Monday's interview that he and his allies have "basically moved on" from the feud.
"I would just say, go back to what you did well. I think it's going to be the right business decision, and all that," he said.
But DeSantis has frequently invoked his battle with Disney on the campaign trail as he seeks the 2024 Republican presidential nomination. The governor, whose political style features a heavy emphasis on fighting "woke" social issues, has accused Disney of sexualizing children.
Iger told CNBC last month that those claims are "preposterous and inaccurate."
Once seen as a major threat to in the GOP primary race, DeSantis has struggled to close his polling gap with the former president and shore up concerns from some top donors about the campaign.
Disclosure: NBCUniversal is the parent company of Universal Studios and CNBC.
— CNBC's Sarah Whitten contributed to this report.
